NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. Temozolomide (TMZ), an alkylating agent, is widely used for treating primary and recurrent high-grade gliomas. What does hydroxychloroquine sulphate do Plaquenil yeast infection Chloroquine maculopathy screening Mar 16, 2018 In vivo studies have demonstrated that metformin and chloroquine can pass through the BBB appreciably. However, high levels of metformin efflux transporters have been reported in glioma and this raises concerns regarding the intratumoral bioavailability of metformin Molenaar et al. 2017. Sorafenib and chloroquine cooperated to inhibit the glioma cell viability. Human glioma cell lines LN229 black line and U373 grey line were plated in 96-well plates 5×10 3 /well, and treated with sorafenib alone 10 µM and in combination with 5 µM chloroquine for 24, 48 and 72 hours. Cell viability was examined by CCK-8 assay. In vitro, U373 cells engineered to overexpress EGFR were more sensitive to chloroquine treatment than unaltered U373 glioblastoma cells. Other publications by this group showed an increased dependence of EGFR overexpressing cancer cells on autophagy, while chloroquine is a known autophagy inhibitor. Recently, studies have found that TMZ treatment could induce autophagy, which contributes to therapy resistance in glioma. However, the efficacy of TMZ is often limited by the development of resistance. Glioma chloroquine in vivo In vitro and in vivo antitumor effects of chloroquine on oral., Inhibition of Autophagy by Chloroquine Enhances the. Deschloroquine used on chloroquine resistant parasites Jan 27, 2010 Activation of the p53 growth suppression/apoptotic pathway is one of the promising strategies in targeting glioma cells. We show that the quinoline derivative chloroquine activates the p53 pathway and suppresses growth of glioma cells in vitro and in vivo in an orthotopic U87MG human glioblastoma mouse model. Chloroquine activates the p53 pathway and induces apoptosis.. Repurposed Drugs Astrocytoma Options. In vitro and in vivo antitumor effects of chloroquine on.. Moreover, the PI3K-mTOR inhibitor NVP-BEZ235, which is in clinical use, synergized with the lysosomotropic inhibitor of autophagy, chloroquine, another agent in clinical use, to induce apoptosis in glioma xenografts in vivo, providing a therapeutic approach potentially translatable to humans. For instance, some of in vitro research data laid a foundation to initiate clinical trials such as data showing that chloroquine treatment might create benefits for glioma patients, since it may sensitize glioma cells to ionizing radiation via decreasing viable hypoxic fraction of tumor cells and autophagy. All above, these data support that NTZ exhibits anti-glioma properties in vivo. Fig. 6 NTZ inhibits glioma growth in vivo. a Dissected tumors from a xenograft model with or without 27-day NTZ.